Author
Year
Title
Journal
Method
Exercise
Protocol
Type of
Exercise
Type of
Interven
-
tion
Category
Zafei
-
ridis et
al.
2016
Global metabolic stress of isoeffort
continuous and high intensity inter
-
val aerobic
exercise: a comparative
1H RMN metabonomic study.
J Proteome
Res
NMR
Continuous training x
short (30 s) and long (3
min) HIIT in a station
-
ary bicycle
Aerobic
Acute
1
Berton
et al.
2016
Metabolic time-course response
after resistance exercise: A metabo
-
lomics approach
J Sports Sci
NMR
Four sets of 10 repeti
-
tions at 70% 1RM in
45º leg press and leg
extension machine
Strength
Acute
1
Danaher
et al.
2016
The use of metabolomics to monitor
simultaneous changes in metabolic
variables following supramaximal
low volume high-intensity exercise
Metabolo
-
mics
GC –
MS
150% VO2max HIIT x
300% VO2max HIIT in
a stationary bicycle
Aerobic
Acute
1
Muhsen
et al.
2016
-
imal Exercise at a Standardised
Relative Intensity in Healthy Adults
Metabolites
LC –
MS
Submaximal protocol in
a stationary bicycle
Aerobic
Acute
1
Hansen
et al.
2015
Type 2 diabetes alters metabolic
and transcriptional signatures of
glucose and amino acid metabolism
during exercise and recovery.
Diabetologia
HPLC –
MS
60 min protocol at 50%
of the Vo2max in a
stationary bicycle
Aerobic
Acute
4
Glynn et
al.
2015
Impact of combined resistance
and aerobic exercise training
on branched-chain amino acid
turnover, glycine metabolism and
insulin sensitivity in overweight
humans
Diabetologia
GC –
MS
Aerobic exercise 3-4 x/
week at 65-80% of the
VO2max and strength
exercise in 8 different
exercise equipment
Aero
-
bic and
Strength
Chronic
4
Kuehn
-
baum et
al.
2015
Multiplexed separations for bio
-
marker discovery in metabolomics:
Elucidating adaptive responses to
exercise training
Electropho
-
resis
MS
– CE –
MS
6-week HIIT, 2x/week,
ten 60 s sprints at 90%
of the VO2max
Aerobic
Chronic
1
Wang et
al.
2015
Applying
1H RMN Spectroscopy
to Detect Changes in the Urinary
Metabolite Levels of Chinese Half-
Pipe Snowboarders after Different
Exercises
J Anal
Methods
Chem
NMR
Abdominal strength
exercises and exercises
for snowboarding
Aerobic
Acute
1
Ma et al.
2015
The intervention effects of acu
-
puncture on fatigue induced by
exhaustive physical exercises: A
metabolomics investigation
Evid Based
Complement
Alternat Med
NMR
8 km of running and 16
100 m sprints
Aerobic
Acute
3
Daskala
-
ki et al.
2015
A Study of the Effects of Exercise
on the Urinary Metabolome Using
Normalisation to Individual Meta
-
bolic Output
Metabolites
LC –
MS
50 min of aerobic
exercise (cycling and
running)
Aerobic
Acute
1
Pech
-
livanis
et al.
2015
Monitoring the response of the
human urinary metabolome to brief
maximal exercise by a combination
of RP-UPLC-MS and
1H RMN
spectroscopy
J Proteome
Res
UPLC –
MS and
NMR
Running (sprints) with
short and long intervals
between stimuli
Aerobic
Acute
1
Nieman
et al.
2014
-
-
mation, oxidative stress, immune
dysfunction, and metabolite shifts
in cyclists: A randomized, crossover
trial
PLoS ONE
UHPLC
– MS
and
GC–
MS
75 km cycling
Aerobic
Acute
3
Jacobs
et al.
2014
Metabolic response to decaffeinat
-
ed green tea extract during rest and
moderate-intensity exercise
J Agric
Food Chem
GC –
MS and
LC –
MS
30 min stationary
bicycle at 50% of the
maximum power
Aerobic
Acute
2
Kuehn
-
baum et
al.
2014
Personalized metabolomics for pre
-
dicting glucose tolerance changes in
sedentary women after high-intensi
-
ty interval training
Sci Rep
MS
– CE –
MS
6-week HIIT, 18 ses
-
sions, 3x/week, ten 60
s sprints
Aerobic
Chronic
4
Nieman
et al.
2014
Metabolomics approach to assess
-
ing plasma 13 – and 9-hydroxy-oc
-
tadecadienoic acid and linoleic
acid metabolite responses to 75-km
cycling
Am J Physi
-
ol Regul
Integr Comp
Physiol
UHPLC
– MS
and
GC–
MS
75 km cycling
Aerobic
Acute
3
Mukher
-
jee et al.
2014
Whole blood transcriptomics and
adaptive biochemical pathways of
high-intensity exercise in 50-60 year
old masters athletes
PLoS ONE
NMR
Submaximal endurance
high-intensity exercise
in a stationary bicycle,
1 h in a ramp up to 90%
of the VO2max
Aerobic
Acute
1
Santone
et al.
2014
Saliva metabolomics by RMN for
the evaluation of sport performance
J Pharm
Biomed
Anally
NMR
Yo-Yo Test in soccer
players
Aerobic
Acute
3
Reinehr
et al.
2014
Changes in the serum metabolite
weight loss
Eur J Nutr
HPLC –
MS
Dances, games, and
races
Aerobic
Chronic
4
Peake et
al.
2014
Metabolic and hormonal respons
-
es to isoenergetic high-intensity
interval exercise and continuous
moderate-intensity exercise
Am J Physi
-
ol Endocri
-
nol Metab
GC –
MS
HIIT at 80% of the
VO2max x Continuous
moderate exercise at
65% of the VO2max in
a stationary bicycle
Aerobic
Acute
1
Huffman
et al.
2014
Metabolite signatures of exercise
training in human skeletal muscle
relate to mitochondrial remodeling
Diabetologia
GC –
MS
Aerobic and resistance
exercise of low, medi
-
um and high intensity
Aero
-
bic and
Strength
Chronic
1
Sheedy
et al.
2014
1H-RMN analysis of the human
urinary metabolome in response
to an 18-month multi-component
exercise program and calcium–vi
-
tamin-D3
supplementation in older
men
Appl
Physiol Nutr
Metab
NMR
Multi-component exer
-
cise program
Chronic
2
Ra et al.
2014
Metabolomics of salivary fatigue
markers in soccer players after
consecutive games
Appl Physiol
Nutr Metab
ESI –
MS
Soccer games
Soccer
Acute
3
Nieman
et al.
2013
Serum metabolic signatures
exercise period persist after 14 h of
recovery in runners
J Proteome
Res
UHPLC
– MS
and
GC–
MS
2.5 h of running during
three days at 70% of the
VO2max
Aerobic
Acute
3
Nieman
et al.
2013
Protein Powder on Exercise-In
-
Stress in Athletes: A Randomized
Trial Using a Metabolomics Ap
-
proach
PLoS ONE
UHPLC
– MS
and GC
– MS
2.5 h of running during
three days at 70% of the
VO2max
Aerobic
Acute
3
Yde et
al.
2013
Metabonomic Response to Milk
Proteins after a Single Bout of
Heavy Resistance Exercise Elu
-
cidated by
1H Nuclear Magnetic
Resonance Spectroscopy
Metabolites
NMR
10 x 8 reps. in a uni
-
lateral leg extension
machine
Strength
Acute
2
Neal et
al.
2013
Six weeks of a polarized train
-
ing-intensity distribution leads to
greater physiological and perfor
-
mance adaptations than a threshold
model in trained cyclists
J Appl
Physiol
NMR
Six weeks of training
for cyclists
Aerobic
Chronic
3
Pech
-
livanis
et al.
2013
1H RMN study on the short-and
long-term impact of two training
programs of sprint running on the
serum
J Proteome
Res
NMR
Running sprints
Aerobic
Acute/
Chronic
1
Hodgson
et al.
2013
Metabolic response to green tea
extract during rest and moderate-in
-
tensity exercise
J Nutr Bio
-
chem
GC –
MS and
LC –
MS
60 min of 50% Max
-
imum Power in a
stationary bicycle
Aerobic
Acute
2
Schubert
et al.
2012
Metabolic monitoring and assess
-
ment of anaerobic threshold by
means of breath biomarkers
Metabolo
-
mics
PTR –
MS
Maximum effort
protocol in a stationary
bicycle
Aerobic
Acute
1
Li et al.
2012
Independent component analysis in
non-hypothesis driven metabolom
-
ics: Improvement of pattern discov
-
data interpretation demonstrated
with plasma samples of exercising
humans
J Chromato
-
gr B Analyt
Technol
Biomed Life
Sci
GC –
MS
Two hours in a uni
-
lateral leg extension
machine
Strength
Acute
1
Brugnara
et al.
2012
Metabolomics approach for analyz
-
ing the effects of exercise in sub
-
jects with type 1 diabetes mellitus
PLoS ONE
NMR
and GC
– MS
30 min in a stationary
bicycle at 80% of the
VO2max
Aerobic
Acute
4
Krug et
al.
2012
The dynamic range of the human
metabolome revealed by challenges
FASEB
Journal
Fia –
MS,
PTR –
MS and
NMR
30 min in the anaerobic
threshold of each in a
stationary bicycle
Aerobic
Acute
1
Netzer
et al.
2011
physical exercise: A computation
-
and kinetic analysis of metabolic
biomarkers
J Clin Bio
-
informa
MS –
MS
Progressive exercise
in a stationary bicycle
with an increase of 25
W/min until exhaustion
Aerobic
Acute
1
Pech
-
livanis
et al.
2010
1H RMN-based metabonomic
investigation of the effect of two
different exercise sessions on the
urine
J Proteome
Res
NMR
Running sprints
Aerobic
Acute
1
Lehmann
et al.
2010
Medium Chain Acylcarnitines
Dominate the Metabolite Pattern in
Humans under Moderate Inten
-
sity Exercise and Support Lipid
Oxidation
Plos OnE
UPLC –
MS
60 min continuous
running at 75% of the
VO2max
Aerobic
Acute
1
Bruce et
al.
2010
approach applied to an exercise
study monitoring the effects of glu
-
cose, galactose, and fructose drinks
during post-exercise recovery
J Chromato
-
gr B Analyt
Technol
Biomed Life
Sci
GC –
MS
HIIT: 2 min at 50%
of the VO2max and 2
min at 90%. When not
reaching it, it decreases
to 80%, then to 70%
until exhaustion.
Aerobic
Acute
2
Lee et al.
2010
Differential metabolomics for
quantitative assessment of oxidative
stress with strenuous exercise and
nutritional intervention: Thiol-spe
-
-
olism with N-acetyl-L-cysteine
pretreatment
Analytical
Chemistry
CE
– ESI –
MS
45 min in a stationary
bicycle at 75% of the
VO2max and then at
90% of the VO2max
until exhaustion
Aerobic
Acute
2
Enea et
al.
2010
1H RMN-based metabolomics
approach for exploring urinary me
-
and chronic physical exercise
Anal Bioanal
Chem
NMR
Short: 30 s sprint at
maximum speed in
a stationary bicycle /
Long: cycling at 75%
of the VO2max until
exhaustion
Aerobic
Acute
1
Miccheli
et al.
2009
the postexercise metabolism of elite
athletes as investigated by RMN-
based metabolomics
J Am Coll
Nutr
NMR
20 min warm-up, 1000
m at maximum intensity
and 50 min in submax
-
imal intensity in a
rowing machine.
Aerobic
Acute
2
Kirwan
et al.
2009
Spectroscopic correlation analysis
of RMN-based metabonomics in
exercise science
Anal Chim
Acta
NMR
70% of the VO2max
until exhaustion in a
rowing machine
Aerobic
Acute
2
Chorell
et al.
2009
Predictive metabolomics evaluation
of nutrition-modulated metabolic
stress responses in human blood se
-
rum during the early recovery phase
of strenuous physical exercise
J Proteome
Res
GC –
MS
90 min, with nine
rounds of 10 min each,
with no intervals: 2 min
at 40%, 8 min at 60%
and 2 min at 85% of the
VO2max
Aerobic
Acute
2
Yan et al.
2009
Metabolomic investigation into
variation of endogenous metabo
-
lites in professional athletes subject
to strength-endurance training
J Appl
Physiol
GC –
MS
Two weeks of technical
and aerobic training for
rowers
Aerobic
Chronic
3
Kuhl et
al.
2008
Metabolomics as a tool to evaluate
exercise-induced improvements in
insulin sensitivity
Metabolo
-
mics
GC –
MS
Aerobic and strength
exercise during 12
weeks, 3 x/week for
50 min
Com
-
bined
Chronic
4
Pohjanen
et al.
2007
A multivariate screening strategy
for investigating metabolic effects
of strenuous physical exercise in
human serum
J Proteome
Res
GC –
MS
90 min, with nine
rounds of 10 minutes
each, with no intervals:
2 min at 40%, 8 min at
60% and 2 min at 85%
of the VO2max
Aerobic
Acute
1